Enhancer‐mediated reporter gene expression in <i>Arabidopsis thaliana</i> : a forward genetic screen

SUMMARY Gene expression is controlled and regulated by interactions between cis ‐regulatory DNA elements (CREs) regulatory proteins. Enhancers are one of the most important classes CREs in eukaryotes. Eukaryotic genes, especially those related to development or responses environmental cues, often multiple enhancers different tissues and/or at developmental stages. Remarkably, little known about molecular mechanisms which regulate gene plants. We identified a distal enhancer, CREβ , regulates AtDGK7 encodes diacylglycerol kinase Arabidopsis. developed transgenic line containing luciferase reporter ( LUC ) driven fused with minimal cauliflower mosaic virus (CaMV) 35S promoter. The enhancer was shown play role response osmotic pressure gene. A forward genetic screen pipeline based on established generate mutations associated altered suite mutants variable levels as well segregation patterns populations. demonstrate that this will allow us identify trans‐ factors function acting regulation endogenous